Review



f tractin green fluorescent protein gfp plasmid  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Addgene inc f tractin green fluorescent protein gfp plasmid
    F Tractin Green Fluorescent Protein Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+f+tractin+egfp/pEGFP-C1+F-tractin-EGFP+(Plasmid+%2358473)/pmc12757058-239-15-22
    Average 94 stars, based on 37 article reviews
    f tractin green fluorescent protein gfp plasmid - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Derivative Assay:

    Article Title: Detection of fluorescent protein mechanical switching in cellulo.
    Article Snippet: .. F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #58473).32 The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021).30 Linker 2 (GGSGSDPPVAT) was used previously in another construct containing F-tractin.33 Gibson Assembly (with Gibson Assembly Master Mix, E2611S; NEB, Ipswich, MA) was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat #: R3101S; NEB)/NotI-HF (Cat #: R3189S; NEB) and the following three fragments containing complementary regions: (1) F-tractin and Linker 1, (2) TSMod, and (3) Linker 2 and F-tractin. ..

    Article Title: Detection of Fluorescent Protein Mechanical Switching in Cellulo
    Article Snippet: .. F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #5847) . .. The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021) .

    Article Title: Detection of fluorescent protein mechanical switching in cellulo
    Article Snippet: .. F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #58473). .. The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021).

    Plasmid Preparation:

    Article Title: Detection of fluorescent protein mechanical switching in cellulo.
    Article Snippet: .. F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #58473).32 The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021).30 Linker 2 (GGSGSDPPVAT) was used previously in another construct containing F-tractin.33 Gibson Assembly (with Gibson Assembly Master Mix, E2611S; NEB, Ipswich, MA) was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat #: R3101S; NEB)/NotI-HF (Cat #: R3189S; NEB) and the following three fragments containing complementary regions: (1) F-tractin and Linker 1, (2) TSMod, and (3) Linker 2 and F-tractin. ..

    Article Title: A tug of war between filament treadmilling and myosin induced contractility generates actin rings
    Article Snippet: Cells tested negative for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza). .. pEGFP-C1 F-tractin-EGFP was a gift from Dyche Mullins (Addgene plasmid # 58473) ( ). ..

    Article Title: Kidney epithelial cells are active mechano-biological fluid pumps.
    Article Snippet: .. For live-cell imaging and measurement of actin dynamics, MDCK-II cells were transiently transfected using pEGFP-C1 F-tractin-EGFP which was a gift from Dr. Dyche Mullins (UCSF) (Addgene plasmid # 58473). ..

    Article Title: Detection of Fluorescent Protein Mechanical Switching in Cellulo
    Article Snippet: .. F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #5847) . .. The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021) .

    Article Title: Detection of fluorescent protein mechanical switching in cellulo
    Article Snippet: .. F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #58473). .. The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021).

    Article Title: Extracellular Hydraulic Resistance Enhances Cell Migration
    Article Snippet: MATLAB and ImageJ were used for all image analysis. .. For retrograde flow measurements, the cells were transiently transfected using pEGFP‐C1 F‐tractin‐EGFP which was a gift from Dyche Mullins Lab (Addgene plasmid # 58473). ..

    Article Title: A tug of war between filament treadmilling and myosin induced contractility generates actin rings
    Article Snippet: Cells tested negative for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza). .. pEGFP- C1 F- tractin- EGFP was a gift from Dyche Mullins (Addgene plasmid # 58473) (Belin et al., 2014). ..

    Construct:

    Article Title: Detection of fluorescent protein mechanical switching in cellulo.
    Article Snippet: .. F-tractin and Linker 1 were derived from pEGFP-C1 F-tractin-EGFP (Addgene Plasmid #58473).32 The original TSMod was derived from pcDNA3.1-TSMod (Addgene Plasmid #26021).30 Linker 2 (GGSGSDPPVAT) was used previously in another construct containing F-tractin.33 Gibson Assembly (with Gibson Assembly Master Mix, E2611S; NEB, Ipswich, MA) was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat #: R3101S; NEB)/NotI-HF (Cat #: R3189S; NEB) and the following three fragments containing complementary regions: (1) F-tractin and Linker 1, (2) TSMod, and (3) Linker 2 and F-tractin. ..

    Transfection:

    Article Title: Cytoskeletal activation of NHE1 regulates cell volume and DNA methylation
    Article Snippet: To maintain stable knockdown, cells transduced with the pLKO.1 puro backbone or sgBag6 1 with Ef1alpha Puro-P2A-BFP(1)(1) backbone were cultured in medium containing 0.5 μg/ml Puromycin (Gibco). .. pEGFP-C1 F-tractin-EGFP (Addgene 58473, a gift from Dyche Mullins) plasmids were used for transient transfections. .. About 60-80% confluent 3T3 or HT1080 cells were transfected using Lipofectamine 3000 reagent following the manufacturer’s recommendations. pHAGE-RSV-GCaMP6s (Addgene 80146, a gift from Darrell Kotton) plasmids were used for lentiviral cell transduction.

    Article Title: Kidney epithelial cells are active mechano-biological fluid pumps.
    Article Snippet: .. For live-cell imaging and measurement of actin dynamics, MDCK-II cells were transiently transfected using pEGFP-C1 F-tractin-EGFP which was a gift from Dr. Dyche Mullins (UCSF) (Addgene plasmid # 58473). ..

    Article Title: Extracellular Hydraulic Resistance Enhances Cell Migration
    Article Snippet: MATLAB and ImageJ were used for all image analysis. .. For retrograde flow measurements, the cells were transiently transfected using pEGFP‐C1 F‐tractin‐EGFP which was a gift from Dyche Mullins Lab (Addgene plasmid # 58473). ..

    Live Cell Imaging:

    Article Title: Kidney epithelial cells are active mechano-biological fluid pumps.
    Article Snippet: .. For live-cell imaging and measurement of actin dynamics, MDCK-II cells were transiently transfected using pEGFP-C1 F-tractin-EGFP which was a gift from Dr. Dyche Mullins (UCSF) (Addgene plasmid # 58473). ..



    Similar Products

    94
    Addgene inc f tractin green fluorescent protein gfp plasmid
    F Tractin Green Fluorescent Protein Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+f+tractin+egfp/pEGFP-C1+F-tractin-EGFP+(Plasmid+%2358473)/pmc12757058-239-15-22
    Average 94 stars, based on 1 article reviews
    f tractin green fluorescent protein gfp plasmid - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc f tractin egfp
    F Tractin Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+f+tractin+egfp/pEGFP-C1+F-tractin-EGFP+(Plasmid+%2358473)/bio_rxiv__2025__11__17__688923-176-4-19
    Average 94 stars, based on 1 article reviews
    f tractin egfp - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    92
    Addgene inc pegfp c1 f tractin egfp addgene plasmid
    Pegfp C1 F Tractin Egfp Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+f+tractin+egfp/pEGFP-C1+Utr230-EGFP+(Plasmid+%2358472)/bio_rxiv__2025__07__22__666217-56-19-21
    Average 92 stars, based on 1 article reviews
    pegfp c1 f tractin egfp addgene plasmid - by Bioz Stars, 2026-09
    92/100 stars
      Buy from Supplier

    94
    Addgene inc dr dyche mullins at ucsf
    a , A plot of BLTP2 mRNA expression score and survival score for ~1,000 cancer cell lines with a deletion of BLTP2 . Data from DepMap. b , Crystal violet-stained colony forming units of HeLa cells plated at the cell number indicated on the top (left). Growth rate of WT and BLTP2 -KO cells ( n = 3) in DMEM with cFBS (right); mean cell number ± s.e.m.; P values from unpaired two-tailed t -tests. c , The AlphaFold predicted secondary structure of yeast Fmp27 (green) and human BLTP2 (magenta) compared and aligned using the Matchmaker plugin of <t>UCSF</t> Chimera. Scale bar, 30 Å. d , Serial dilutions of yeast strains spotted on SC without EtN supplementation and grown for 3 days. e , Western blot (left) of genomically expressed Fmp27-3xHA and Por1 (control). Cells were grown in SC and Fmp27-3xHA and Por1 were immunoprecipitated. Quantification of Fmp27–GFP normalized to Por1 (right). The results are shown as a box–violin plot (the solid bar shows the median and the dotted bar the quartiles); P values from unpaired two-tailed t -tests, n = 3. f , A schematic of Kennedy and CDP-DAG pathways. Created with BioRender.com . g , Serial dilutions yeast strains were spotted on SC without or with 4 mM Cho or 4 mM EtN and grown at 18 °C for 3 days (top). Doubling time of strains grown at 18 °C, with or without EtN supplementation, in a 96-well plate; histograms show mean ± s.e.m. ( n = 3); P values from unpaired two-tailed t -tests (bottom). h , The relative amount of the five major PLs in cells grown at 18 °C and labelled to steady-state with [ 3 H]palmitate; mean ± s.e.m. ( n = 3), P values from two-way ANOVA. i , Serial dilution of yeast strains spotted on SC medium without or with Cho, EtN or PpN and incubated at 23 °C for 3 days. j , The structure of l -[3- 3 H]serine; T indicates the position of tritium (top) and a scheme of PS, PE and PC production by exogenous [ 3 H]serine (top). The bar graph shows the relative abundance of radiolabelled PLs after growth for 1 h at 18 °C in SC (bottom); mean ± s.e.m. ( n = 3); P values from two-way ANOVA. k , The chemical structures of EtN and PpN.
    Dr Dyche Mullins At Ucsf, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+f+tractin+egfp/pEGFP-C1+F-tractin-EGFP+(Plasmid+%2358473)/pmc12270902-442-7-12
    Average 94 stars, based on 1 article reviews
    dr dyche mullins at ucsf - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc f tractin gfp
    a , A plot of BLTP2 mRNA expression score and survival score for ~1,000 cancer cell lines with a deletion of BLTP2 . Data from DepMap. b , Crystal violet-stained colony forming units of HeLa cells plated at the cell number indicated on the top (left). Growth rate of WT and BLTP2 -KO cells ( n = 3) in DMEM with cFBS (right); mean cell number ± s.e.m.; P values from unpaired two-tailed t -tests. c , The AlphaFold predicted secondary structure of yeast Fmp27 (green) and human BLTP2 (magenta) compared and aligned using the Matchmaker plugin of <t>UCSF</t> Chimera. Scale bar, 30 Å. d , Serial dilutions of yeast strains spotted on SC without EtN supplementation and grown for 3 days. e , Western blot (left) of genomically expressed Fmp27-3xHA and Por1 (control). Cells were grown in SC and Fmp27-3xHA and Por1 were immunoprecipitated. Quantification of Fmp27–GFP normalized to Por1 (right). The results are shown as a box–violin plot (the solid bar shows the median and the dotted bar the quartiles); P values from unpaired two-tailed t -tests, n = 3. f , A schematic of Kennedy and CDP-DAG pathways. Created with BioRender.com . g , Serial dilutions yeast strains were spotted on SC without or with 4 mM Cho or 4 mM EtN and grown at 18 °C for 3 days (top). Doubling time of strains grown at 18 °C, with or without EtN supplementation, in a 96-well plate; histograms show mean ± s.e.m. ( n = 3); P values from unpaired two-tailed t -tests (bottom). h , The relative amount of the five major PLs in cells grown at 18 °C and labelled to steady-state with [ 3 H]palmitate; mean ± s.e.m. ( n = 3), P values from two-way ANOVA. i , Serial dilution of yeast strains spotted on SC medium without or with Cho, EtN or PpN and incubated at 23 °C for 3 days. j , The structure of l -[3- 3 H]serine; T indicates the position of tritium (top) and a scheme of PS, PE and PC production by exogenous [ 3 H]serine (top). The bar graph shows the relative abundance of radiolabelled PLs after growth for 1 h at 18 °C in SC (bottom); mean ± s.e.m. ( n = 3); P values from two-way ANOVA. k , The chemical structures of EtN and PpN.
    F Tractin Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+f+tractin+egfp/pEGFP-C1+F-tractin-EGFP+(Plasmid+%2358473)/pm40544224-451-5-6
    Average 94 stars, based on 1 article reviews
    f tractin gfp - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc f tractin egfp construct
    a , A plot of BLTP2 mRNA expression score and survival score for ~1,000 cancer cell lines with a deletion of BLTP2 . Data from DepMap. b , Crystal violet-stained colony forming units of HeLa cells plated at the cell number indicated on the top (left). Growth rate of WT and BLTP2 -KO cells ( n = 3) in DMEM with cFBS (right); mean cell number ± s.e.m.; P values from unpaired two-tailed t -tests. c , The AlphaFold predicted secondary structure of yeast Fmp27 (green) and human BLTP2 (magenta) compared and aligned using the Matchmaker plugin of <t>UCSF</t> Chimera. Scale bar, 30 Å. d , Serial dilutions of yeast strains spotted on SC without EtN supplementation and grown for 3 days. e , Western blot (left) of genomically expressed Fmp27-3xHA and Por1 (control). Cells were grown in SC and Fmp27-3xHA and Por1 were immunoprecipitated. Quantification of Fmp27–GFP normalized to Por1 (right). The results are shown as a box–violin plot (the solid bar shows the median and the dotted bar the quartiles); P values from unpaired two-tailed t -tests, n = 3. f , A schematic of Kennedy and CDP-DAG pathways. Created with BioRender.com . g , Serial dilutions yeast strains were spotted on SC without or with 4 mM Cho or 4 mM EtN and grown at 18 °C for 3 days (top). Doubling time of strains grown at 18 °C, with or without EtN supplementation, in a 96-well plate; histograms show mean ± s.e.m. ( n = 3); P values from unpaired two-tailed t -tests (bottom). h , The relative amount of the five major PLs in cells grown at 18 °C and labelled to steady-state with [ 3 H]palmitate; mean ± s.e.m. ( n = 3), P values from two-way ANOVA. i , Serial dilution of yeast strains spotted on SC medium without or with Cho, EtN or PpN and incubated at 23 °C for 3 days. j , The structure of l -[3- 3 H]serine; T indicates the position of tritium (top) and a scheme of PS, PE and PC production by exogenous [ 3 H]serine (top). The bar graph shows the relative abundance of radiolabelled PLs after growth for 1 h at 18 °C in SC (bottom); mean ± s.e.m. ( n = 3); P values from two-way ANOVA. k , The chemical structures of EtN and PpN.
    F Tractin Egfp Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+f+tractin+egfp/pEGFP-C1+F-tractin-EGFP+(Plasmid+%2358473)/pm39939720-293-1-6
    Average 94 stars, based on 1 article reviews
    f tractin egfp construct - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc egfp c1 f tractin egfp
    a , A plot of BLTP2 mRNA expression score and survival score for ~1,000 cancer cell lines with a deletion of BLTP2 . Data from DepMap. b , Crystal violet-stained colony forming units of HeLa cells plated at the cell number indicated on the top (left). Growth rate of WT and BLTP2 -KO cells ( n = 3) in DMEM with cFBS (right); mean cell number ± s.e.m.; P values from unpaired two-tailed t -tests. c , The AlphaFold predicted secondary structure of yeast Fmp27 (green) and human BLTP2 (magenta) compared and aligned using the Matchmaker plugin of <t>UCSF</t> Chimera. Scale bar, 30 Å. d , Serial dilutions of yeast strains spotted on SC without EtN supplementation and grown for 3 days. e , Western blot (left) of genomically expressed Fmp27-3xHA and Por1 (control). Cells were grown in SC and Fmp27-3xHA and Por1 were immunoprecipitated. Quantification of Fmp27–GFP normalized to Por1 (right). The results are shown as a box–violin plot (the solid bar shows the median and the dotted bar the quartiles); P values from unpaired two-tailed t -tests, n = 3. f , A schematic of Kennedy and CDP-DAG pathways. Created with BioRender.com . g , Serial dilutions yeast strains were spotted on SC without or with 4 mM Cho or 4 mM EtN and grown at 18 °C for 3 days (top). Doubling time of strains grown at 18 °C, with or without EtN supplementation, in a 96-well plate; histograms show mean ± s.e.m. ( n = 3); P values from unpaired two-tailed t -tests (bottom). h , The relative amount of the five major PLs in cells grown at 18 °C and labelled to steady-state with [ 3 H]palmitate; mean ± s.e.m. ( n = 3), P values from two-way ANOVA. i , Serial dilution of yeast strains spotted on SC medium without or with Cho, EtN or PpN and incubated at 23 °C for 3 days. j , The structure of l -[3- 3 H]serine; T indicates the position of tritium (top) and a scheme of PS, PE and PC production by exogenous [ 3 H]serine (top). The bar graph shows the relative abundance of radiolabelled PLs after growth for 1 h at 18 °C in SC (bottom); mean ± s.e.m. ( n = 3); P values from two-way ANOVA. k , The chemical structures of EtN and PpN.
    Egfp C1 F Tractin Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+f+tractin+egfp/pEGFP-C1+F-tractin-EGFP+(Plasmid+%2358473)/pmc11871582-417-0-2
    Average 94 stars, based on 1 article reviews
    egfp c1 f tractin egfp - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc 103 addgene
    a , A plot of BLTP2 mRNA expression score and survival score for ~1,000 cancer cell lines with a deletion of BLTP2 . Data from DepMap. b , Crystal violet-stained colony forming units of HeLa cells plated at the cell number indicated on the top (left). Growth rate of WT and BLTP2 -KO cells ( n = 3) in DMEM with cFBS (right); mean cell number ± s.e.m.; P values from unpaired two-tailed t -tests. c , The AlphaFold predicted secondary structure of yeast Fmp27 (green) and human BLTP2 (magenta) compared and aligned using the Matchmaker plugin of <t>UCSF</t> Chimera. Scale bar, 30 Å. d , Serial dilutions of yeast strains spotted on SC without EtN supplementation and grown for 3 days. e , Western blot (left) of genomically expressed Fmp27-3xHA and Por1 (control). Cells were grown in SC and Fmp27-3xHA and Por1 were immunoprecipitated. Quantification of Fmp27–GFP normalized to Por1 (right). The results are shown as a box–violin plot (the solid bar shows the median and the dotted bar the quartiles); P values from unpaired two-tailed t -tests, n = 3. f , A schematic of Kennedy and CDP-DAG pathways. Created with BioRender.com . g , Serial dilutions yeast strains were spotted on SC without or with 4 mM Cho or 4 mM EtN and grown at 18 °C for 3 days (top). Doubling time of strains grown at 18 °C, with or without EtN supplementation, in a 96-well plate; histograms show mean ± s.e.m. ( n = 3); P values from unpaired two-tailed t -tests (bottom). h , The relative amount of the five major PLs in cells grown at 18 °C and labelled to steady-state with [ 3 H]palmitate; mean ± s.e.m. ( n = 3), P values from two-way ANOVA. i , Serial dilution of yeast strains spotted on SC medium without or with Cho, EtN or PpN and incubated at 23 °C for 3 days. j , The structure of l -[3- 3 H]serine; T indicates the position of tritium (top) and a scheme of PS, PE and PC production by exogenous [ 3 H]serine (top). The bar graph shows the relative abundance of radiolabelled PLs after growth for 1 h at 18 °C in SC (bottom); mean ± s.e.m. ( n = 3); P values from two-way ANOVA. k , The chemical structures of EtN and PpN.
    103 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+f+tractin+egfp/pEGFP-C1+F-tractin-EGFP+(Plasmid+%2358473)/pm39579355-251-63-64
    Average 94 stars, based on 1 article reviews
    103 addgene - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    a , A plot of BLTP2 mRNA expression score and survival score for ~1,000 cancer cell lines with a deletion of BLTP2 . Data from DepMap. b , Crystal violet-stained colony forming units of HeLa cells plated at the cell number indicated on the top (left). Growth rate of WT and BLTP2 -KO cells ( n = 3) in DMEM with cFBS (right); mean cell number ± s.e.m.; P values from unpaired two-tailed t -tests. c , The AlphaFold predicted secondary structure of yeast Fmp27 (green) and human BLTP2 (magenta) compared and aligned using the Matchmaker plugin of UCSF Chimera. Scale bar, 30 Å. d , Serial dilutions of yeast strains spotted on SC without EtN supplementation and grown for 3 days. e , Western blot (left) of genomically expressed Fmp27-3xHA and Por1 (control). Cells were grown in SC and Fmp27-3xHA and Por1 were immunoprecipitated. Quantification of Fmp27–GFP normalized to Por1 (right). The results are shown as a box–violin plot (the solid bar shows the median and the dotted bar the quartiles); P values from unpaired two-tailed t -tests, n = 3. f , A schematic of Kennedy and CDP-DAG pathways. Created with BioRender.com . g , Serial dilutions yeast strains were spotted on SC without or with 4 mM Cho or 4 mM EtN and grown at 18 °C for 3 days (top). Doubling time of strains grown at 18 °C, with or without EtN supplementation, in a 96-well plate; histograms show mean ± s.e.m. ( n = 3); P values from unpaired two-tailed t -tests (bottom). h , The relative amount of the five major PLs in cells grown at 18 °C and labelled to steady-state with [ 3 H]palmitate; mean ± s.e.m. ( n = 3), P values from two-way ANOVA. i , Serial dilution of yeast strains spotted on SC medium without or with Cho, EtN or PpN and incubated at 23 °C for 3 days. j , The structure of l -[3- 3 H]serine; T indicates the position of tritium (top) and a scheme of PS, PE and PC production by exogenous [ 3 H]serine (top). The bar graph shows the relative abundance of radiolabelled PLs after growth for 1 h at 18 °C in SC (bottom); mean ± s.e.m. ( n = 3); P values from two-way ANOVA. k , The chemical structures of EtN and PpN.

    Journal: Nature Cell Biology

    Article Title: The Vps13-like protein BLTP2 regulates phosphatidylethanolamine levels to maintain plasma membrane fluidity and breast cancer aggressiveness

    doi: 10.1038/s41556-025-01672-3

    Figure Lengend Snippet: a , A plot of BLTP2 mRNA expression score and survival score for ~1,000 cancer cell lines with a deletion of BLTP2 . Data from DepMap. b , Crystal violet-stained colony forming units of HeLa cells plated at the cell number indicated on the top (left). Growth rate of WT and BLTP2 -KO cells ( n = 3) in DMEM with cFBS (right); mean cell number ± s.e.m.; P values from unpaired two-tailed t -tests. c , The AlphaFold predicted secondary structure of yeast Fmp27 (green) and human BLTP2 (magenta) compared and aligned using the Matchmaker plugin of UCSF Chimera. Scale bar, 30 Å. d , Serial dilutions of yeast strains spotted on SC without EtN supplementation and grown for 3 days. e , Western blot (left) of genomically expressed Fmp27-3xHA and Por1 (control). Cells were grown in SC and Fmp27-3xHA and Por1 were immunoprecipitated. Quantification of Fmp27–GFP normalized to Por1 (right). The results are shown as a box–violin plot (the solid bar shows the median and the dotted bar the quartiles); P values from unpaired two-tailed t -tests, n = 3. f , A schematic of Kennedy and CDP-DAG pathways. Created with BioRender.com . g , Serial dilutions yeast strains were spotted on SC without or with 4 mM Cho or 4 mM EtN and grown at 18 °C for 3 days (top). Doubling time of strains grown at 18 °C, with or without EtN supplementation, in a 96-well plate; histograms show mean ± s.e.m. ( n = 3); P values from unpaired two-tailed t -tests (bottom). h , The relative amount of the five major PLs in cells grown at 18 °C and labelled to steady-state with [ 3 H]palmitate; mean ± s.e.m. ( n = 3), P values from two-way ANOVA. i , Serial dilution of yeast strains spotted on SC medium without or with Cho, EtN or PpN and incubated at 23 °C for 3 days. j , The structure of l -[3- 3 H]serine; T indicates the position of tritium (top) and a scheme of PS, PE and PC production by exogenous [ 3 H]serine (top). The bar graph shows the relative abundance of radiolabelled PLs after growth for 1 h at 18 °C in SC (bottom); mean ± s.e.m. ( n = 3); P values from two-way ANOVA. k , The chemical structures of EtN and PpN.

    Article Snippet: The Ftractin-EGFP construct was a gift from Dr. Dyche Mullins at UCSF (Addgene plasmid 58473) and MDA-MB-231 cells were a gift from Dr. Rolf Brekken (UT Southwestern Medical Center).

    Techniques: Expressing, Staining, Two Tailed Test, Western Blot, Control, Immunoprecipitation, Serial Dilution, Incubation